Fig. 7
From: Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions
Live vs. heat-killed S. uberis strain 0140J. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at ~ 50,000 BMMOs/well. BMMOs were challenged with live or heat-killed (30 min at 63 °C) S. uberis strain 0140J at a multiplicity of infection (MOI) of 50:1 S. uberis strain 0140 J:BMMO. Supernatants were collected 4 h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were also unstimulated in a no treatment group and this mean was deducted from the other values, which were then standardised to the LPS positive control (10 ng/mL). Data is presented as mean ± SD with N = 3 (normally assumed) and statistically analysed using an unpaired t-test (ns = not significant)