Fig. 6

S. uberis MOI of BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at ~ 50,000 BMMOs/well. BMMOs were challenged with heat-killed S. uberis strain 0140 J at a multiplicity of infection (MOI) of 1:1, 10:1, 50:1 and 100:1 S. uberis strain 0140 J:BMMO. Supernatants were collected at 20, 22 and 24 h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were also unstimulated in a no treatment group and this mean was deducted from the other values, which were then standardised to the LPS positive control (10 ng/mL) at 20 h. Data is presented as mean of this ratio ± SD with N = 6. Shapiro-Wilk determined the data to be normally distributed and so was statistically analysed using a two-way ANOVA with Tukey’s multiple comparison post hoc test (***P < 0.001 and **P < 0.01 compared to 50:1 at the corresponding time; ns = not significant)