Fig. 4
From: Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions
IL-1β produced from BMMOs over 24 h following challenge with S. uberis strain 0140J. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at ~ 50,000 BMMOs/well. BMMOs were challenged with heat-killed S. uberis strain 0140J at a multiplicity of infection (MOI) of 50:1 0140 J:BMMO. Supernatants were collected and the concentration of IL-1β was measured by ELISA every 2 h over a 24 h period. Data is presented as mean ± SD with N = 6