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Table 1 Encapsulation efficiency and mole ratio of VP37-dsRNA with the three variants of MrN-VLPs

From: Interior modification of Macrobrachium rosenbergii nodavirus-like particle enhances encapsulation of VP37-dsRNA against shrimp white spot syndrome infection

Type of VLP

dsRNA encapsulation

Amount of dsRNA (µg)a

Percent encapsulationb

Average mole number

Mole ratio (dsRNA/VLP)

V0- MrN-VLP

5.22 ± 3.57

52.22

0.97 × 10− 12

7.48 ± 0.51

V1-MrN-VLP

6.72 ± 3.18c

67.21

1.24 × 10− 12

9.63 ± 0.46

V2 MrN-VLP

7.37 ± 4.09c

73.68

1.36 × 10− 12

10.55 ± 0.59

  1. aDetermined by densitometric analysis of dsRNA’s band intensity in a agarose gel electrophoresis against the standard curve of a known amount of dsRNA (from 3–15 µg)
  2. bPercent encapsulation = the estimated amount of dsRNA/10 µg×100, where 10 µg is the starting amount of dsRNA used in encapsulation experiments
  3. Letter c = statistical differences between the V0, V1 and V2 groups
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BMC Veterinary Research

ISSN: 1746-6148

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