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Table 3 Posterior median and 95% credible interval (CI95) for a conditional independent test for the determination of sensitivity (Se), specificity (Sp), and prevalence (Prev) of three Mycoplasma bovis antibody ELISA tests (ID-screen≥ 50%, Bio K302, Bio K432) on 156 bulk tank milk samples from Belgian and Swiss dairy herds, while using different cutoff values for the sample-to-positive percentage (S/P%) of the Bio K302 and Bio K432. Optimal cutoff values and results for Bio K302 and Bio K432 are highlighted bold

From: Diagnostic performance of Mycoplasmopsis bovis antibody ELISA tests on bulk tank milk from dairy herds

Test

S/P% cutoff

Se (%) (CI95)

Sp (%) (CI95)

Prev (%) (CI95)

Youden’s

index (J)

Bio K302

≥ 50%

59.6 (34.7–88.2)

88.1 (80.2–95.3)

20.1 (9.0-38.7)

0.48

 

≥ 40%

67.0 (43.6–92.1)

82.5 (73.4–91.6)

23.4 (11.1–41.8)

0.50

 

≥ 37%

66.8 (43.2–92.0)

79.3 (69.9–88.6)

22.2 (9.71–41.3)

0.46

 

≥ 30%

76.7 (58.2–94.7)

77.5 (67.1–90.3)

30.9 (18.1–47.6)

0.54

 

≥ 20%*

58.6 (45.5–68.8)

13.5 (2.6–28.7)

69.5 (50.2–88.6)

-0.28

Bio K432

≥ 50%

74.8 (47.0-96.1)

99.3 (96.2–100.0)

19.3 (8.0-37.8)

0.29

 

≥ 40%

37.4 (18.2–74.5)

97.7 (93.0-99.8)

22.2 (9.7–41.3)

0.35

 

≥ 30%

65.3 (37.6–95.3)

93.3 (87.0-98.1)

18.7 (9.7–34.6)

0.59

 

≥ 20%

89.8 (67.2–99.5)

74.7 (64.9–84.9)

22.5 (13.2–37.6)

0.65

 

≥ 10%**

-

-

-

-

  1. * Results for BIO K302 cutoff value of ≥ 20% were obtained by using ‘generation inits’
  2. ** Model unidentifiable