Identification of neuropathogenic Varicellovirus equidalpha1 as a potential cause of respiratory disease outbreaks among horses in North Xinjiang, China, from 2021-2023

Table 1 Primers and probe for EqAHVs in this study

Primer	Sequence (5´-3´)	Product size (bp)	Reference
EqAHV-1 Forward Primer	AATACCATGGACCACCGTTGA	58
EqAHV-1 Reverse Primer	TCCACCCGCCGCTCTT	58
EqAHV-1-Probe	FAM-CATAATCATCCGCTCAAAT-MGB
EqAHV-1 ORF33 Forward Primer1	ATGTCCTCTGGTTGCCGTTCTG	1644	[16]
EqAHV-1 ORF33 Reverse Primer1	CTCGTTTGTGCAAGCGATGTAG	1644	[16]
EqAHV-1 ORF33 Forward Primer2	GTGAGATCTAACCGCACCTACGAC	1463	[16]
EqAHV-1 ORF33 Reverse Primer2	TTAAACCATTTTTTCATYTTCCATG	1463	[16]
EqAHV-1 ORF30 Forward Primer	CGGAGTAAGGCTTGTGGTTTCG	559	[7, 16]
EqAHV-1 ORF30 Reverse Primer	GTGGGCTACCAGGGAGCAAAG	559	[7, 16]
EqAHV-4 ORF33 Forward Primer	GGGTCCTACAGATTTACTATTCG	587	[7]
EqAHV-4 ORF33 Reverse Primer	ACACCACGCAGTTGCTATCCTAC	587	[7]
EqAHV-5 ORF8 Forward Primer	TGTATCACCGTGGACCAAGAGAG	881	[7, 24]
EqAHV-5 ORF8 Reverse Primer	TCAAAGATGGATCTTCTCTGAGTG	881	[7, 24]
EqAHV-2 ORF8 Forward Primer	GGTGACACTATAGAGATGTCRCC	716	[7, 24]
EqAHV-2 ORF8 Reverse Primer	CTGTTGATGCTCTTTCTGAGATTG	716	[7, 24]

ISSN: 1746-6148