Table 1 Primers sequences used for qRT-PCR.
Name | Accession number | Sequences (5′–3′) | Product,bp |
|---|---|---|---|
GFAP forward | NM_017009.2 | AGAGGAAGGTTGAGTCGCTGGAG | 145 |
GFAP reverse | AGAGCCGCTGTGAGGTCTGG | ||
IBA1 forward | NM_017196.3 | AGCGAATGCTGGAGAAACTTGGG | 84 |
IBA1reverse | CCTCGGAGCCACTGGACACC | ||
NeuN forward | NM_001134498.3 | CCACCACTCTCTTGTCCGTT | 165 |
NeuN reverse | ATCAGCAGCCGCATAGACTC | ||
Bcl-2 forward | NM_016993.2 | GAACTGGGGGAGGATTGTGG | 80 |
Bcl-2 reverse | GGGGTGACATCTCCCTGTTG | ||
Bax forward | NM_017059.2 | GTCCTCACTGCCTCACTCAC | 189 |
Bax reverse | GTTTATTGGCACCTCCCCCA | ||
GAPDH forward | NM_001394060.2 | TTCCTACCCCCAATGTATCCG | 270 |
GAPDH reverse | CCACCCTGTTGCTGTATCCATA | ||
CCND1 forward | NM_001379248.1 | GAGGCGGATGAGAACAAGCAGATC | 96 |
CCND1 reverse | GGAGGGTGGGTTGGAAATGAACTTC | ||
CD86 forward | NM_019388.3 | TCTGCCGTGCCCATTTACAAAGG | 104 |
CD86 reverse | TGCCCAAATAGTGCTCGTACAGAAC | ||
iNOS forward | NM_001313922.1 | CAGCTGGGCTGTACAAACCTT | 95 |
iNOS reverse | CATTGGAAGTGAAGCGTTTCG | ||
Arg-1 forward | NM_007482.3 | AACCTTGGCTTGCTTCGGAACTC | 131 |
Arg-1 reverse | GTTCTGTCTGCTTTGCTGTGATGC | ||
TNF-α forward | NM_001278601.1 | ACGCTCTTCTGTCTACTGAACTTCG | 113 |
TNF-α reverse | TGGTTTGTGAGTGTGAGGGTCTG | ||
IL-6 forward | NM_001314054.1 | ACTTCCAGCCAGTTGCCTTCTTG | 110 |
IL-6 reverse | TGGTCTGTTGTGGGTGGTATCCTC |