Table 3 The effect of breed, sex and their interactions on fatty acid composition, IMF and FMP of Bowen Genetics Forest Pastoral Angus, Hereford and Wagyu beef cattle studs

Intramuscular fat (%)

 Breed (Hereford vs Angus)

0.50

1.41

0.73

 Breed (Wagyu vs Angus)

-2.57

1.26

0.05

 Sex (Male vs Female)

-7.03

1.53

 < 0.01

 Breed*Sex

2.07

1.85

0.27

Fat melting point (°C)

 Breed (Hereford vs Angus)

15.43

1.70

 < 0.01

 Breed (Wagyu vs Angus)

-17.12

1.50

 < 0.01

 Sex (Male vs Female)

-12.36

1.82

 < 0.01

 Breed*Sex

17.09

2.30

 < 0.01

Oleic acid (18:1n-9)

 Breed (Hereford vs Angus)

-70.62

339.81

0.84

 Breed (Wagyu vs Angus)

477.35

298.19

0.11

 Sex (Male vs Female)

-392.58

368.92

0.29

 Breed*Sex

-399.46

441.68

0.37

LA¹ (18:2n-6)

 Breed (Hereford vs Angus)

-0.75

5.12

0.88

 Breed (Wagyu vs Angus)

13.64

4.50

 < 0.01

 Sex (Male vs Female)

0.41

5.56

0.94

 Breed*Sex

-3.94

6.66

0.56

ALA² (18:3n-3)

 Breed (Hereford vs Angus)

0.00

1.37

1.00

 Breed (Wagyu vs Angus)

5.10

1.21

 < 0.01

 Sex (Male vs Female)

0.75

1.49

0.62

 Breed*Sex

-3.59

1.79

0.05

EPA³ (20:5n-3)

 Breed (Hereford vs Angus)

0.32

0.22

0.16

 Breed (Wagyu vs Angus)

0.93

0.20

 < 0.01

 Sex (Male vs Female)

0.03

0.24

0.92

 Breed*Sex

-0.64

0.29

0.03

DHA⁴ (22:6n-3)

 Breed (Hereford vs Angus)

0.19

1.73

0.91

 Breed (Wagyu vs Angus)

7.87

1.52

 < 0.01

 Sex (Male vs Female)

0.86

1.88

0.65

 Breed*Sex

-7.12

2.25

 < 0.01

DPA⁵ (22:5n-3)

 Breed (Hereford vs Angus)

0.32

1.55

0.84

 Breed (Wagyu vs Angus)

6.04

1.36

 < 0.01

 Sex (Male vs Female)

0.78

1.69

0.65

 Breed*Sex

-4.22

2.02

0.04

EPA + DHA

 Breed (Hereford vs Angus)

0.51

3.21

0.88

 Breed (Wagyu vs Angus)

13.91

2.82

 < 0.01

 Sex (Male vs Female)

1.63

3.49

0.64

 Breed*Sex

-11.34

4.18

0.01

EPA + DHA + DPA

 Breed (Hereford vs Angus)

-0.25

6.83

0.97

 Breed (Wagyu vs Angus)

27.55

6.00

 < 0.01

 Sex (Male vs Female)

2.05

7.42

0.78

 Breed*Sex

-15.28

8.88

0.09

ALA + EPA + DHA + DPA

 Breed (Hereford vs Angus)

-2.89

357.01

0.99

 Breed (Wagyu vs Angus)

236.80

313.29

0.45

 Sex (Male vs Female)

-367.70

387.60

0.34

 Breed*Sex

134.51

462.35

0.77

tSFA⁶

 Breed (Hereford vs Angus)

-78.43

419.72

0.85

 Breed (Wagyu vs Angus)

368.21

368.32

0.32

 Sex (Male vs Female)

-465.10

455.68

0.31

 Breed*Sex

71.53

543.56

0.90

tMUFA⁷

 Breed (Hereford vs Angus)

-6.33

28.11

0.82

 Breed (Wagyu vs Angus)

64.76

24.67

0.01

 Sex (Male vs Female)

5.84

30.52

0.85

 Breed*Sex

-47.35

36.41

0.20

tPUFA⁸

 Breed (Hereford vs Angus)

-7.74

22.37

0.73

 Breed (Wagyu vs Angus)

38.28

19.63

0.05

 Sex (Male vs Female)

6.54

24.28

0.79

 Breed*Sex

-38.85

28.97

0.18

n-6 PUFA⁹

 Breed (Hereford vs Angus)

2.07

7.71

0.79

 Breed (Wagyu vs Angus)

26.71

6.77

 < 0.01

 Sex (Male vs Female)

-0.55

8.37

0.95

 Breed*Sex

-9.81

9.98

0.33

n-3 PUFA¹⁰

 Breed (Hereford vs Angus)

-0.32

0.21

0.13

 Breed (Wagyu vs Angus)

-0.52

0.18

0.01

 Sex (Male vs Female)

0.15

0.23

0.50

 Breed*Sex

-0.30

0.27

0.27

n-6/n-3¹¹

 Breed (Hereford vs Angus)

0.06

0.03

0.09

 Breed (Wagyu vs Angus)

0.10

0.03

 < 0.01

 Sex (Male vs Female)

-0.04

0.04

0.34

 Breed*Sex

0.05

0.04

0.25