Table 1 Validation parameters of optimized HPLC-MS/MS method
Fish type | Calibration curve | Salinomycin concentration (ng/ml) | recovery (%, n = 3) | Intraday RSD (%, n = 5) | Interday RSD (%, n = 3) |
|---|---|---|---|---|---|
Olive flounder | y = 6522.04 x + 9991.27 | 5 | 89.01 | 1.95 | 13.1 |
y = 6492.71 x + 11408.9 | 20 | 82.93 | 9.17 | - | |
y = 6579.44 x + 9017.85 | 40 | 80.27 | 1.49 | - | |
Black rockfish | y = 8087.59 x + 19659.1 | 5 | 97.1 | 7.48 | 10.5 |
y = 8201.38 x + 6546.1 | 20 | 96.3 | 0.99 | - | |
y = 8169.09 x + 4464.11 | 40 | 91.1 | 4.01 | - |