Addition of low concentration of cholesterol-loaded cyclodextrin (CLC) has a positive effect on cryopreserved canine spermatozoa evaluated by andrological and biophysical methods

Table 4 The effect of HBCD and CLC on the anisotropy (ANISO) and general polarization (GP) of cryopreserved canine sperm plasma membranes compared with the control group (n = 11). All data are presented as mean ± sd. Given p-value is for repeated measures ANOVA (A) or for Friedman test (F). Groups that differed statistically significantly from controls are marked with * (p-value < 0.05)

	CONTROL	0.83 mg/ml CLC	1.66 mg/ml CLC	3.32 mg/ml CLC	1.66 mg/ml HBCD	p-value
ANISO 23 °C	0.109 ± 0.003	0.122 ± 0.014*	0.111 ± 0.003	0.123 ± 0.017*	0.115 ± 0.007*	0.00108 (F)
ANISO 38 °C	0.079 ± 0.004	0.081 ± 0.004	0.082 ± 0.004	0.081 ± 0.005	0.081 ± 0.004	0.784 (F)
GP 23 °C	0.460 ± 0.027	0.478 ± 0.023	0.457 ± 0.017	0.474 ± 0.018	0.483 ± 0.021	0.025 (A)
GP 38 °C	0.455 ± 0.030	0.462 ± 0.037	0.456 ± 0.035	0.477 ± 0.031	0.480 ± 0.027	0.0509 (F)

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