Table 3 The effect of HBCD and CLC on the sperm membrane integrity (SYBR-14/PI), sperm acrosome status (PNA), sperm mitochondrial activity (JC-1/PI), apoptosis and membrane lipid disorder (YO-PRO-1/M540) and sperm chromatin structure status (AO) in the canine cryopreserved sperm compared with the control group (n = 11). All data are presented as mean ± sd. Given p-value is for repeated measures ANOVA (A) or for Friedman test (F). Groups that differed statistically significantly from controls are marked with * (p-value < 0.05)
| Â | CONTROL | 0.83Â mg/ml CLC | 1.66Â mg/ml CLC | 3.32Â mg/ml CLC | 1.66Â mg/ml HBCD | p-value |
|---|---|---|---|---|---|---|
LIVE (%) | 51.24 ± 18.27 | 51.82 ± 13.17 | 33.50 ± 11.67 | 42.34 ± 12.82 | 37.39 ± 15.59 | 0.005 (A) |
L/IACR (%) | 36.51 ± 9.48 | 40.09 ± 8.49 | 33.84 ± 10.60 | 33.16 ± 8.51 | 33.67 ± 9.70 | 0.232 (A) |
HMMP (%) | 55.98 ± 16.04 | 65.19 ± 12.91 | 56.55 ± 18.44 | 46.95 ± 14.17 | 50.09 ± 16.39 | 0.00163 (F) |
L/STPM (%) | 39.29 ± 7.24 | 43.46 ± 9.62 | 40.30 ± 8.79 | 36.41 ± 8.04 | 34.01 ± 10.92 | 0.056 (A) |
DFI (%) | 3.57 ± 0.77 | 3.58 ± 1.18 | 4.55 ± 1.44 | 4.53 ± 2.10 | 4.20 ± 0.66 | 0.650 (F) |