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Table 1 The effect of HBCD and CLC on the sperm motility parameters of frozen-thawed canine semen compared with the control group (n = 11). All data are presented as mean ± sd. Given p-value is for repeated measures ANOVA (A) or for Friedman test (F). Groups that differed statistically significantly from controls are marked with * (p-value < 0.05)

From: Addition of low concentration of cholesterol-loaded cyclodextrin (CLC) has a positive effect on cryopreserved canine spermatozoa evaluated by andrological and biophysical methods

 

CONTROL

0.83 mg/ml CLC

1.66 mg/ml CLC

3.32 mg/ml CLC

1.66 mg/ml HBCD

p-value

MOT (%)

46.1 ± 17.7

57.5 ± 11.4

45.4 ± 13.8

48.1 ± 10.2

33.4 ± 10.3

0.0549 (F)

PMOT (%)

21.7 ± 11.9

31.2 ± 14.1*

22.7 ± 11.5

24.3 ± 11.3

13.6 ± 11.7*

0.0008 (A)

RAP (%)

24.2 ± 13.9

34.4 ± 16.2*

24.7 ± 13.2

27.3 ± 13.5

14.9 ± 13.2*

0.001 (A)

SLOW (%)

17.1 ± 10.3

19.5 ± 8.7

18.3 ± 8.4

17.4 ± 7.6

15.7 ± 6.5

0.198 (F)

ELONG (%)

60.4 ± 2.2

60.2 ± 1.7

59.9 ± 1.7

59.7 ± 1.8

59.8 ± 2.6

0.651 (F)

STR (%)

123.4 ± 20.1

128.6 ± 18.2

122.2 ± 14.6

126.3 ± 15.5

109.6 ± 24.5

0.051 (A)

LIN (%)

110.8 ± 16.2

116.6 ± 16.3

110.5 ± 11.7

113.1 ± 12.9

100.7 ± 22.0

0.101 (A)

VAP (µm/s)

177.6 ± 30.3

179.8 ± 26.4

174.2 ± 25.6

182.4 ± 24.0

152.0 ± 38.8

0.028 (A)

VSL (µm/s)

6.8 ± 0.8

6.7 ± 0.9

6.7 ± 0.9

7.0 ± 0.7

6.2 ± 0.9

0.0825 (F)

VCL (µm/s)

26.8 ± 3.9

27.1 ± 4.2

6.7 ± 0.9

26.8 ± 3.8

25.5 ± 4.1

0.688 (A)

ALH (µm/s)

88.5 ± 1.8

89.3 ± 2.4

89.1 ± 2.3

88.0 ± 2.2

90.3 ± 1.9

0.099 (A)

BCF (Hz)

63.1 ± 3.2

65.1 ± 5.4

64.5 ± 5.6

62.6 ± 4.2

66.8 ± 4.6

0.144 (A)