Table 1 Biochemical characteristics of V. alginolyticus isolates retrieved from gilthead sea bream

Growth on TCBS

Large yellow colony

Swarming on blood agar and TSA

+

Growth at 28 C

+

Growth at 37 C

+

Growth at 0% NaCl

-

Growth at 2% NaCl

+

Growth at 10% NaCl

+

Gram stain

Gram -ve short rods

Motility on soft agar

+

Cytochrome oxidase

+

Catalase

+

Indole

+

Voges-Proskauer (VP)

-

Citrate utilization

-

Methyl red (MR)

+

Urea hydrolysis

-

Triple sugar iron (TSI)

K/A – A/A

Carbohydrate fermentation:

Sucrose

+

Glucose

+

Lactose

-

Mannose

+

Mannitol

+

Sorbitol

-

Arabinose

-

Maltose

+

Rhammose

-

Melibiose

-

Inositol

-

Nitrate reduction

+

Hydrogen sulfide (H₂S)

-

Esculin hydrolysis

-

Gelatin hydrolysis

-

Arginine dihydrolase

-

Ornithine decarboxylase

+

Lysine decarboxylase

+

Tryptophane production

+