Table 2 Primers for plasmid construction
From: The role of IBV PL1pro in virus replication and suppression of host innate immune responses
plasmids | Primers | Sequences (5′ -3 ′) |
|---|---|---|
pcDNA5-FLAG-PL1pro-N | F | CGCGGATCCCTGCCTCTTGATGAAGAT |
R | CCGCTCGAGTTATTCTTGCGCATCAACAG | |
pKTO-IBV-ΔPL1pro | F | TAAATACCCTCCAGCCACATGTGAGAAA |
R | ATGTGGCTGGAGGGTATTTAATACTTGC | |
pKTO-IBV-ΔN-PL1pro | F | TAAATACCCTCAACTGTGTCAACAAGAG |
R | GACACAGTTGAGGGTATTTAATACTTGC |