Fig. 6
From: The role of IBV PL1pro in virus replication and suppression of host innate immune responses
Absence of the N-terminal region of PL1pro up-regulates the expressions of IFIT family members during later stages of infection. (a) mRNA expressions of ISGs in rIBV and rIBV-ΔPL1pro-N infected cells. H1299 cells were stimulated with IFN-α (100ng/ml) at 6 h post-infection, and qRT-PCR was performed to analyze the mRNA expressions of IFIT1 and IFIT2. Data were presented as mean ± SD (N = 3). *P < 0.05. The raw data is presented in Additional file 2. (b) Protein expressions of ISGs in rIBV and rIBV-ΔPL1pro-N infected cells. H1299 cells were stimulated with IFN-α (100ng/ml) at 6 h post-infection, Western blot was performed to analyze the protein expressions of IFIT1 and IFIT2. The full-length blots are in Additional file 1: Supplemental Fig. 6