Fig. 2
From: The role of IBV PL1pro in virus replication and suppression of host innate immune responses
Recovery of rIBV, rIBV-ΔPL1pro, and rIBV-ΔPL1pro-N from cells electroporated with in vitro-synthesized full-length transcripts. (a) A schematic of the genome in nsp3 of rIBV, rIBV-ΔPL1pro, and rIBV-ΔPL1pro-N. (b) In vitro transcription of the full-length transcripts of rIBV, rIBV-ΔPL1pro, and rIBV-ΔPL1pro-N. Lanes 2–6,8–12,14–18: The five cDNA fragments covering IBV sequences from plasmids pKTO-IBV-A (pKTO-IBV-ΔPL1pro, pKTO-IBV-ΔPL1pro-N), pGEM-IBV-B, pXL-IBV-C, pGEM-IBV-D, and pGEM-IBV-E, respectively; Lanes 20–22: In vitro assembly of the five fragments into a full-length cDNA of rIBV, rIBV-ΔPL1pro, and rIBV-ΔPL1pro-N; Lanes 24–26: Generation of the full-length in vitro transcripts of rIBV, rIBV-ΔPL1pro, and rIBV-ΔPL1pro-N; Lanes 1, 7, 13, 19 and 23: DNA markers. The full-length gels are in Additional file 1: Supplemental Fig. 2. (c) CPE in Vero cells electroporated with in vitro-synthesized transcripts derived from the assembled full-length clones of rIBV, rIBV-ΔPL1pro, and rIBV-ΔPL1pro-N. Scale bars: 50 μm