Table 2 Gene specific sequences primers used in real-time quantitative PCR
Target genes | Primers and probes sequence (5’ → 3’) |
|---|---|
NOD1 | AGGAGCTCTCATCAGCGAAGATCT |
GCAGCGTCAGCAGAAGACCATT | |
TLR2 | CCTGGTGTTCGTGTTCATCCTCAT |
AGTTGGAGTCCTTCTCACTGTAGG | |
TLR4 | ACGGATGGCTTTGGTTGGGATTA |
GATGTAGCTATCTGGTGCTTGGAAT | |
MDA5 | CAGCGAGTTGCCCTAGCCTCA |
AACACCTCCCTTCGCCCGTCT | |
DAI | CACGCGGTTGGTGCTATCATTG |
ATACTGCCTTGTGCCTCGAACTG | |
LGP2 | CCAGAATGAGCAGCAGGAC |
AATGTTGCACTCAGGGATGT | |
MAVS | CCTGACTCAAACAAGGGAAG |
AATCAGAGCGATGCCAACAG | |
P50 | CAGCCAACTGGGAGGTGTAT |
GCTGCTTGGAGCTTTCATTC | |
P65 | GTGTGAAGAAACGGGAACTG |
GGCACGGTTGTCATAGATGG | |
RelB | GTGCTGTCTGAGCCCATCTTCG |
TACGGCGGCGTTCGG | |
GAPDH | GTGGTGGCCATCAATGATCC |
ACTTGTGATCAATGGGCACG |