Morphologic, phenotypic, and genotypic similarities between primary tumors and corresponding 3D cell cultures grown in a repeatable system—preliminary results

Table 2 NGS data of primary tumors and 3D cell cultures. In bold: ratio MDM2/TP53 suggestive of MDM2 amplification

Case	Specimen	TP53	MDM2	Mean coverage (reads per amplicon)	Mean coverage MDM2	Mean coverage TP53	Ratio (MDM2/TP53)
1	Primary Tumor	WT	WT	1112	1685	157	10.7
1	3D cell culture	WT	WT	613	990	60	16.5
2	Primary Tumor	WT	WT	1105	1185	1000	1.2
2	3D cell culture	WT	WT	144	151	132	1.1
3	Primary Tumor	WT	WT	1763	1947	1565	1.2
3	3D cell culture	WT	WT	711	806	590	1.4

ISSN: 1746-6148