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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Morphologic, phenotypic, and genotypic similarities between primary tumors and corresponding 3D cell cultures grown in a repeatable system—preliminary results

Fig. 3

Immunophenotype of primary mesenchymal tumor (perivascular wall tumor) and the respective 3D cultures, diaminobenzidine. (a) Perivascular wall tumor, vimentin: solid whorls of neoplastic cells with intense diffuse cytoplasmic immunoexpression. (b) Perivascular wall tumor, actin: negative expression by neoplastic cells and internal positive control in the vessel wall. (c) Perivascular wall tumor, calponin: negative expression by neoplastic cells. (d) 3D culture of perivascular wall tumors, vimentin: solid whorls of neoplastic cells with intense diffuse cytoplasmic immunoexpression in the periphery of the aggregate and less intense labeling in the internal portion penetrant in the scaffold. (e) 3D culture of perivascular wall tumor, actin: negative expression by neoplastic cells. (f) 3D culture of perivascular wall tumor, calponin: negative expression by neoplastic cells

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