Fig. 3
From: Resolvin D1 alleviates apoptosis triggered by endoplasmic reticulum stress in IPEC-J2 cells
Determination of the effective concentration of RvD1 relieving ER stress and apoptosis. (A) The effects of various concentrations of RvD1 (0, 1, 10, 20, 50 nM) on cell viability. (B) The effects of various concentrations of RvD1 (0, 1, 10, 20, 50 nM) on the gene expression of GRP-78. (C) The effects of 1 and 10 nM RvD1 on the gene expression of Caspase-3. (D) The effects of 1 and 10 nM RvD1 on cell morphology. The cell morphology was photographed with an inverted microscope (×100). Different lowercase letters on the graph bars indicate statistically significant differences among the groups (ANOVA with Duncan’s test, p < 0.05). Data are presented as means ± SEM. The analysis of gene expression and cell viability are 3 and 8 replicates in each group, respectively. RvD1, Resolvin D1; Tuni, tunicamycin; GRP-78, glucose-regulated protein 78