Fig. 4

Prokaryotic expression of Bactrian camel TLR8. A Double enzyme digestion results of the TLR8 recombinant plasmid; M DL10000 DNA Marker; 1 pET-28a-TLR8 was digested with BamH I and Xho I; 2 pET-28a (+). B Induced expression results of the TLR8 recombinant plasmid; M Protein molecular weight Marker. The same below; 1 Pre-induction products of recombinant bacteria; 2 Recombinant bacteria induced products. C Optimization of IPTG concentration of the TLR8 recombinant protein; 1 Pre-induction products of recombinant bacteria; 2-7 Recombinant bacteria were induced by IPTG concentration of 0.1 mmol/L, 0.3 mmol/L, 0.5 mmol/L, 0.7 mmol/L, 0.9 mmol/L and 1.0 mmol/L. D Optimization of expression time of the TLR8 recombinant protein; 1 Pre-induction products of recombinant bacteria; 2-6 Products of recombinant bacteria induced for 2 h, 4 h, 6 h, 8 h, and 10 h. E Expression form of the TLR8 recombinant protein; 1 Before ultrasonication of the recombinant bacteria after induction; 2 Lysis supernatant of the recombinant bacteria after induction; 3 Lysis precipitate of the recombinant bacteria after induction. F Purification of the TLR8 recombinant protein; 1 Purified recombinant protein