Table 1 Samples used during rat training and tests. All samples were heat-inactivated prior to behavioral sessions. Brucella-positive samples served as scent targets for rats, while all Brucella-negative samples served as non-reinforced controls
From: Scent detection of Brucella abortus by African giant pouched rats (Cricetomys ansorgei)
Sample | Brucella | Experiment | Description |
|---|---|---|---|
5+ | Positive | Exp 2: Novel trials only | 5-day old Brucella culture isolates with nutrient agar |
7+ | Positive | Exp 1, 2, and 3 | 7-day old Brucella culture isolates with nutrient agar |
10+ | Positive | Exp 1, 2, and 3 | 10-day old Brucella culture isolates with nutrient agar |
10BA+ | Positive | Exp 1: Phase I only | 10-day old Brucella culture isolates with blood agar media |
12+ | Positive | Exp 1, 2, and 3 | 12-day old Brucella culture isolates with nutrient agar |
7- | Negative | Exp 1, 2, and 3 | Blank nutrient agar incubated for 7 days |
10- | Negative | Exp 1, 2, and 3 | Blank nutrient agar incubated for 10 days |
12- | Negative | Exp 1, 2, and 3 | Blank nutrient agar heat inactivated after 12-day period |
E. Coli | Negative | Exp 1, 2, and 3 | E. coli culture in nutrient agar |
5BA- | Negative | Exp 2: Novel trials only | 5-day old blood agar media |
Feces+ | Positive | Exp 3 | fecal sample spiked with 10-day old Brucella -positive solution |
Feces- | Negative | Exp 3 | fecal sample spiked with 10-day old Brucella -negative solution |