Fig. 3
Sensitivity of the three methods. A Sensitivity of the standard RPA for nucleic acid detection. After amplification at 39 °C for 15 min, 5 μl of each reaction was electrophoresed (2% agarose), M: 500 bp Marker (Takara, Code No. 3590Q), 1–8 corresponds to 108–100 copies/μl plasmid standard, N: a negative control. B Sensitivity of the DETECTR method. A volume of 3 μl of standard RPA product was added to the Cas12a cleavage system, and maximum fluorescent signals were recorded (n = 3, error bars show the mean ± SEM). C Sensitivity of the CRISPR/CAST package, 1–8 corresponding to plasmid copy numbers of 107–100 copies/μl, N: negative control, the green arrow is the quality control line, and the orange arrow is the detection line