Fig. 1
From: Usability of serum hedgehog signalling proteins as biomarkers in canine mammary carcinomas
Comparison of SHH and GLI-1 expression in the normal mammary gland and MGT tissues. A For western blotting, the blots were cut prior to hybridisation with antibodies. SHH (35 kDa) and GLI-1 (100–140 kDa) protein levels in the normal mammary gland and benign and malignant mammary gland tissues. Beta-actin was used as the loading control. B Relative quantitation of SHH and (C) GLI-1 proteins were normalized to that of the control group. The mean and standard error of the mean are used to express quantitative data. (*, **, #: p < .05). SHH, sonic hedgehog; GLI-1, glioma-associated oncogene 1; MGT, mammary gland tumour