Table 6 Primer sequences (5’ to 3’) used to amplify the gene ahaI in A. hydrophila, yielding a 200 bp amplicon
Primer | Primers sequences (5’-3’) | Tm (°C) | Reference |
|---|---|---|---|
ahaI Forward | 5- GAGAAGGTGACCACCAAGAACA-3 | 57.8 | (58) |
ahaI Reverse | 5- GAGATGTCAGCCTTGTAGAGCT-3 | 54.2 |