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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge

Fig. 2

Plasmid maps and construction results, phenotypic characteristics of vaccine candidate strains. (A) Plasmid maps of pYA3493 and pS-PlpE. (B) Identification of recombinant plasmid pS-PlpE by PCR and restriction enzyme digestion. M: DL2000 or DL5000 DNA marker; Lane 1, 2, 3: EcoR I and Sal I digested pS-PlpE (different clones). (C) The expression of PlpE in rSC0016 was analyzed by WB. Lane 1: purified PlpE protein; lane 2: BL21(pET-28a). (D) Plasmid stability of pS-PlpE during passage. M: DL5000 DNA marker; Lane 1: 10 passages; lane 2: 20 passages; lane 3: 30 passages; lane 4: 40 passages; lane 5: 50 passages. (E) Growth curves of the rSC0016(pS-PlpE), rSC0016(pYA3493), C78-3 strains in the LB medium

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BMC Veterinary Research

ISSN: 1746-6148

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