Fig. 3

The scores plot of OPLS-DA and permutation tests. A, (E) and (C), (G) Orthogonal partial least square discriminant analysis (OPLS-DA) of scores and permutation test plots for the DS vs HS and TS vs DS samples analyzed in the positive ion mode, respectively. (B), F and (D), (H) Orthogonal partial least square discriminant analysis of scores and permutation test plots for the DS vs HS and TS vs DS samples analyzed in the negative ion mode, respectively. T [1] = first principal component. To [1]  = second orthogonal component. The intercept limit of Q², calculated by regression line, is the plot of Q.² from permutation test in the OPLS-DA model. HS = healthy calf plasma sample; DS = diarrheal calf plasma sample on D0; TS = PLP treatment diarrheal calf plasma sample on D5