Table 1 Experimental conditions for cell and viral culture in CelCradle
Run | Medium (cDMEM) | Cell culture | Medium exchange timing during cell culture | Viral culture | Harvest timing during viral culture | Multiplicity of infection (MOI) |
|---|---|---|---|---|---|---|
Lot A | HyClone DMEM | Semi-batch culture | 4 days post-seeding | Fed-batch culture | 3 days post-infection (dpi) | 0.1 |
Lot B | HyClone DMEM | Semi-batch culture | 2, 4, and 5 days post-seeding | Semi-batch culture | 1, 2, and 3 dpi | 0.1 and 0.01 |
Lot C | HyClone DMEM | Perfusion culture | 5 days post-seeding | Semi-batch culture | 1, 2, and 3 dpi | 0.01 |
Lot D | Welgene DMEM | Semi-batch culture | 2, 4, and 5 days post-seeding | Semi-batch culture | 1 and 2 dpi | 0.01 |