Table 1 Antibodies Used in IHC (Antigen Retrieval, Dilutions, and Sources)
From: First report of primary testicular leiomyosarcoma in two dogs
ANTIBODY | CLONE | SUPPLIER | ANTIGEN RETRIVAL | DILUTION |
|---|---|---|---|---|
Cytokeratin | MNF 116 | Dako, Carpinteria, CA, USA | 30 min. in pH 6.0, citrate buffer, Leica Bound-Max | 1:75 |
Vimentin | 3B4 | Dako, Carpinteria, CA, USA | 30 min. in pH 6.0, citrate buffer, Leica Bound-Max | 1:100 |
Desmin | D33 | ScyTek Laboratories, Inc., USA | 20 min. in pH 9.0, EDTA buffer, Leica Bound-Max | Ready to use |
α-Smooth Muscle Actin | 1A4 | Sigma-Aldrich, USA | 20 min. in pH 6.0, citrate buffer, Leica Bound-Max | 1:1000 |
Smooth Muscle Myosin | SMMS-1 | Sigma-Aldrich, St. Louis, MO. | 20 min. in pH 6.0 citrate buffer, Leica Bound-Max | 1:400 |
Inhibin α | R1 | Serotec Corporation Oxford, UK | MW 650 W, 10 min. in pH 6.0 citrate buffer | 1:40 |
Von Willebrand Factor | Polyclonal | Dako, Carpinteria, CA, USA | 20 min. in pH 9.0, EDTA buffer, Leica Bound-Max | 1:800 |