Table 1 The results (mean ± standard deviation) of measuring different factors, including inflammatory cytokines, acute phase proteins and total antioxidant capacity, and lipid peroxidation index in the serum of dogs in the control group (only laparotomy, n = 6), the 5 min treatment group, laparotomy and exposure of the ovaries with ultrasonic waves for 5 min (n = 10) and 10 min treatment group, laparotomy, and exposure of the ovaries with ultrasonic waves for 10 min (n = 7) in the days before surgery (day 0), and the days after surgery and exposing the ovaries to ultrasonic waves (days 3, 6 and 9)

IL6 (ng/ml)

Control

0.3 ± 0.04 ^A

0.66 ± 0.07^aB

0.37 ± 0.06^aC

0.29 ± 0.04 ^A

T5

0.31 ± 0.04 ^A

1.55 ± 0.1^bB

1.01 ± 0.06^bC

0.31 ± 0.04 ^A

T10

0.29 ± 0.05 ^A

1.63 ± 0.11^cB

1.13 ± 0.06^bC

0.29 ± 0.04 ^A

TNFα (ng/ml)

Control

1.04 ± 0.46^B

2.32 ± 0.85^aA

1.37 ± 0.54^aB

1.03 ± 0.42^B

T5

0.97 ± 0.48 ^A

3.49 ± 0.44^bB

2.18 ± 0.15^bC

1.10 ± 0.47 ^A

T10

1.08 ± 0.47 ^A

3.86 ± 0.41^bB

2.29 ± 0.14^bC

1.10 ± 0.43 ^A

IL1β (pg/ml)

Control

0.1 ± 0.02^B

0.27 ± 0.06^aA

0.14 ± 0.02^aB

0.09 ± 0.03^B

T5

0.1 ± 0.02 ^A

0.54 ± 0.1^bB

0.28 ± 0.08^bC

0.1 ± 0.03 ^A

T10

0.09 ± 0.02 ^A

0.57 ± 0.11^bB

0.29 ± 0.08^bC

0.11 ± 0.03 ^A

SAA (mg/ml)

Control

6.49 ± 0.77^B

15.99 ± 1.85^aA

8.22 ± 1.06^aB

6.35 ± 0.77^B

T5

6.50 ± 0.74 ^A

41.01 ± 4.83^bB

28.5 ± 4.40^bC

6.54 ± 0.77 ^A

T10

6.52 ± 0.67 ^A

45.38 ± 3.41^cB

29.20 ± 3.60^bC

6.59 ± 0.76 ^A

CRP (µg/ml)

Control

3.27 ± 0.45^B

9.11 ± 1.32^aA

4.07 ± 0.32^aB

3.25 ± 0.47^B

T5

3.33 ± 0.43 ^A

30.56 ± 6.53^bB

20.26 ± 3.55^bC

5.29 ± 0.85 ^A

T10

3.34 ± 0.37 ^A

36.66 ± 5.05^cB

20.74 ± 4.39^bC

5.35 ± 1.04 ^A

TAC (mmol/l)

Control

3.18 ± 1.05

2.79 ± 0.86

3.12 ± 0.85

3.18 ± 1.05

T5

3.22 ± 1

2.70 ± 0.61

3.13 ± 0.82

3.20 ± 1.02

T10

3.19 ± 0.85

2.54 ± 0.54

2.96 ± 0.70

3.19 ± 0.82

MDA (µmol/l)

Control

0.57 ± 0.03^B

0.62 ± 0.05^aA

0.57 ± 0.03^aB

0.55 ± 0.02^B

T5

0.55 ± 0.03 ^A

0.77 ± 0.05^bB

0.64 ± 0.05^bC

0.55 ± 0.03 ^A

T10

0.54 ± 0.02 ^A

0.81 ± 0.04^cB

0.65 ± 0.04^bC

0.54 ± 0.03 ^A