Table 2 Summary of the European College of Veterinary Ophthalmology (ECVO) recommended electroretinography (ERG) protocol [1, 22]:
ERG step | Total duration | Cells evaluated | Flash intensity |
|---|---|---|---|
Dark adaptation | 20 min | Rod evaluation: at 0 (10 s after dark adaptation), 4, 8, 12, 16 and 20 min. | 0.01 cd s m-2 |
Dark (scotopic mix) | Immediately after dark adaptation | Mixed rod-cone response evaluation with a single flash | 3 cd s m-2 |
Light adaptation | 10 min | Cone response evaluation with a single flash | 3 cd s m-2 |
Flicker test | Immediately after light adaptation | 3 cd s m-2 |