Efficacy of zinc oxide and copper oxide nanoparticles on virulence genes of avian pathogenic E. coli (APEC) in broilers

Table 1 Primers sequences, target genes, amplicon sizes and cycling conditions for conventional PCR and real time quantitative-PCR

Target gene	Primers sequences	Reverse transcription	Primary denaturation	Amplification (40 cycles)			Reference
Target gene	Primers sequences	Reverse transcription	Primary denaturation	Secondary denaturation	Annealing (Optics on)	Extension	Reference
ibeA	TGGAACCCGCTCGTAATATAC	65˚C/ 60 min.	95˚C/ 15 min.	95˚C/ 15 s.	60˚C/ 1 min.	72˚C/ 30 s.	[19]
ibeA	CTGCCTGTTCAAGCATTGCA						[19]
hlyA	AACAAGGATAAGCACTGTTCTGGC						[21]
hlyA	ACCATATAAGCGGTCATTCCCGTCA						[21]
Iss	ATCACATAGGATTCTGCCG						[22]
Iss	CAGCGGAGTATAGATGCCA						[22]
papC	GTGGCAGTATGAGTAATGACCGTTA						[23]
papC	ATATCCTTTCTGAGGGATGCAATA						[23]
ompA	GGTGTTGCCAGTAACCGG						[19]
ompA	GGTGGTGCACTGGAG TGG						[19]
16 S rRNA	GACCTCGGTTTAGTTCACAGA						[24]
16 S rRNA	CACACGCTGACGCTGACCA						[24]

ISSN: 1746-6148