Fig. 4

Chromosomal sex-determination. Expression of Y- and X-chromosome specific sequences determination by polymerase chain reaction (PCR) (A) and in situ hybridization (ISH) (B). (A) The AMEL and SRY loci detected by PCR in blood. In males, 340 bp of AMEL nonspecific sequence was amplified. PC; positive control, NC; negative control. Negative control used water as the template. (B) Fluorescence-ISH images. X-chromosomal probes labeled with biotination and detected by streptavidin DyLight 549 (red), Y-chromosomal probes were detected by fluorescein-5-isothiocyanate (FITC) (green). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). The white boxed shows higher magnification from the figure. Scale bars = 10 µm