Fig. 2

a. Histopathology sections of the cecum, ileum, and liver of CNP-NS non-supplemented chicks 24 h after S. Enteritidis- challenge. Marked destructive effects on the cecal, ileal, and jejunal mucosa with the influx of a large number of acute inflammatory cells, including heterophils (light blue arrows). The cecal, ileal, and jejunal glands were partially destroyed by the infiltrated inflammatory cells (black arrows). The villous mucosal epithelium was severely damaged and necrotized. The mucosal blood vessels were moderately hyperemic (orange arrow). Intravascular microthrombosis (blue arrows in the window), moderate round cells and heterophils portal infiltration (green arrow), and hepatocellular degeneration (mostly hydropic degeneration) (red arrow) were all seen in the liver. The spleen demonstrates a mild decrease in the germinal lymphocytic population (red arrow), moderate sinusoidal dilatation, and heterophil infiltration (black arrow), as well as focal histiocytic proliferation (light blue arrow). H&E X 100, 400. b. Histopathology sections of the cecum, ileum, and liver of CNP-NS supplemented chicks 24 h after S. Enteritidis-challenge. The histopathological sections show marked influx of lymphocytes (RC, black arrow), while, a few cecal glands appear totally necrotic (CG, green arrow). Ileal tissues have partial epithelial desquamation and mucosal round cells (lymphocytes, plasma cells, and macrophages (yellow and red stars) infiltration. Hepatic tissue shows lymphoplasmacytic infiltrations and aggregations (RC, black arrow), they are also seen in the interstitial tissue without detectable tissue damage (IST) The scale bars are 50 and 100 um