Table 1 Binding site of the last 10 nucleotides of reverse primer, AE982 to positive samples and sequences with highest similarity percentage. First row of the table represents the position of the nucleotide on the CytB gene. Two samples were observed with G/T misbinding on the 3’-end of reverse primers post sequencing whereas twelve samples were observed to have exact sequence with Haemoproteus-specific primer. Possible insertion or deletion at binding site were reconfirmed by sequencing visible amplicons of 1773 bp size under UV light from the first round consensus PCR. Highlighted yellow: AE982 reverse primer sequence from 5’-end; Highlighted blue: sequence at primer binding site of positive samples; Highlighted green: sequence at primer binding site of reference with highest match; Highlighted orange: mismatch nucleotides to primer sequence. Dots ‘⋅’ represents similar nucleotide with primer nucleotide

From: Prevalence of haemosporidia in Asian Glossy Starling with discovery of misbinding of Haemoproteus-specific primer to Plasmodium genera in Sarawak, Malaysian Borneo