Fig. 2

Cropped electrophoresis gel image of A. nested-multiplex PCR amplification of the CytB gene of avian Plasmodium and Haemoproteus using primer set AE980/AE982 and AE983/AAE985 respectively, producing amplicon of 580 bp only; B. nested PCR amplification of the CytB gene of avian Plasmodium using Haemoproteus-specific primer set HaemF/AE982 producing amplicon of 820 bp; C. nested PCR amplification of CytB gene of avian Leucocytozoon using primer set HaemFL/HaemR2L producing amplicon of 523 bp. (see Additional files 3, 4, and 5: Figure S2 – S4). Visualisation of PCR amplifications for nested-multiplex and nested protocol were done on different gels. The last two lanes on the gel contains the negative (water) and positive control for each primer set. Expected band size of amplicons were based on the 100 bp molecular weight ladder, indicated by the bold line. Positive and negative interpretations are indicated below each band