Nutritional and immunological evaluation of Nannochloropsis oculata as a potential Nile tilapia-aquafeed supplement

Table 2 Ingredients and body composition of basal and experimental isocaloric and isonitrogenous diets (%)

Ingredients (g)	Control	N 5	N 10
Yellow corn	19.5	17	16
Soybean meal	20	20	17
Fish meal	20	17	16
Corn gluten	3	3	3
Gelatin	2	2	2
Sunflower oil	3.5	4	4.16
Wheat bran	30.16	30.16	30
Minerals and vitamins premix	1	1	1
Salt	0.3	0.3	0.3
Vitamin C	0.12	0.12	0.12
Dicalcium phosphate	0.1	0.1	0.1
Methionine	0.32	0.32	0.32
*Algae (N. oculata***)	0	5	10
Proximate analysis (% dry matter basis)
Crude Protein^a	32.3	31.4	33.2
Lipid^a	6.69	6.74	6.86
Ash^a	7.75	8.49	7.41
Ca^a	1.17	1.17	1.27
P^a	0.53	0.53	0.57
DE (Digestable Energy)^b (kcal/kg)	3016	3015	3000

The levels of the micro minerals &vitamins for tilapia are covered by supplementation of trace minerals & vitamins premixes as recommended by NRC (2011). Vitamins premix (IU or mg/kg diet); vit. A 5000, Vit.D3 1000, vit. E 20, vit. k3 2, vit. B1 2, vit. B2 5, vit. B6 1.5, vit. B12 0.02, Pantothenic acid 10, Folic acid 1, Biotin 0.15, Niacin 30. Mineral mixture (mg/kg diet); Fe 40, Mn 80, Cu 4, Zn 50, I 0.5, Co 0.2 & Se 0.2
N. oculataNannochloropsis oculata
^aanalyzed
^bcalculated value

ISSN: 1746-6148