Fig. 8

Relative expression of inflammatory markers in mammary glands. Normal lactating mammary glands (Control; blue), and 24 h after challenge with bMSC (orange), LPS (red) or LPS followed by bMSCs six hours thereafter (green) the relative expression of Ly6G (neutrophil marker), KC/Cxcl1, Mip2/Cxcl2, TNFɑ, IL1β, IL6, IL10, iNOS, and A20 genes was quantified. Each data point within box plots represents a single gland and data demonstrates recruitment of neutrophils (Ly6G) and increased expression of inflammatory markers. Each experimental group was compared to the normal control group or between groups and statistical significance was determined by non-parametric Mann–Whitney two-independent-samples test using GraphPad Prism 6 (GraphPad Software, Inc.) and P value of 0.05 or less was considered significant. Non-significant; (ns); P > 0.05, *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001