Fig. 3

The normal diestrus murine uterus. Synchronized diestrus virgin 6–8 weeks old female BALB/C mice were used as normal controls to establish the structure, immune cells composition and expression of inflammatory markers in steady state uteri. Annotated microscopic images of H&E stained FFPE section (A) and cryo sections stained with Phalloidin (B-D). Annotated structural elements include; lumen (L), luminal epithelium (LE), endometrial glands (EG), endometrial stroma (ES), inner muscles (IM), outer muscles (OM), blood vessels (BV), perimetrium (PM) and broad ligament. Immune fluorescence staining was used to demonstrate CD45 + immune cells in the normal diestrus uterus (Ca-d), most of which were F4/80 + macrophages (Da-d). Staining intensities were subjected to nonlinear adjustments so that structures would be visible at low magnification. Scale bars; 200 µm (A), 100 µm (B-Ca), 50 µm (Da) and 20 µm (Cb-d, Db-d)