Fig. 2

Bovine mesenchymal stromal cells (bMSC) suppress activation of NF-kB by LPS in endometrial cells, mammary epithelial cells and macrophages. Bovine endometrial cells (BEND), bovine primary endometrial epithelial cells (PEP), mouse mammary epithelial cell line (Eph4) or mouse macrophages cell line (RAW 264.7) expressing the luminescence NF-kB reporter were co-cultured with bMSC and activated with LPS (A). Fluorescence microscopic images of bMSC stained with DAPI, phalloidin and CFSE are shown in (B). Co-culture experiments were performed at 1:1, 1:10, 1:100 and 1:1000 ratios of bMSC to BEND (C), PEP (D), Eph4 (E) or RAW 264.7 (F) cells. D-luciferin was added at time 0, 50 and 100 min (broken lines) and luminescence signals were quantified every 5 min using SpectraMax i3x multiple detection microplate reader (Molecular Devices, CA USA). Scale bars 20 µm