Fig. 10

Effects of systemic bMSC and mMSC injectionon murine coliform mastitis. Lactating mammary glands were challenged with mammary pathogenic E. coli strain P4-NR bacteria by intramammary infusion. Mice were randomly allocated to the following groups; non-treated (blue), bMSC-treated (red) and mMSC-treated (green). MSC treatment was applied by intravenous infusion 6 h after challenge and all animals were sacrificed 24 h after challenge, mammary tissues were harvested for total bacterial counts and RNA extraction for RT-qPCR analysis. Total bacterial counts were not different between MSC-treated and non-treated animals (A). Box plots (B-L) presenting relative expression of inflammatory markers in mammary tissues of normal lactating animals (Control; black) and experimental groups. Each data point within box plots represents a single gland and each experimental group was compared to the normal control group or between groups by non-parametric Mann–Whitney two-independent-samples test using GraphPad Prism 6 (GraphPad Software, Inc.). P value of 0.05 or less was considered significant. Non-significant; (ns); P > 0.05, *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001