Fig. 1

Activation of inflammatory response in endometrial and mammary epithelial cells. Bovine endometrial cell line (BEND) and mouse mammary alveolar epithelial cell line (Eph4) were transduced with luminescence NF-kB reporter (A-B). Activation of NF-kB, the master regulator of inflammation, in BEND cells is demonstrated in response to pathogen associated molecular patterns (PAMPs); LPS (C), PAM3CSK4 (D) and poly I:C (E). Activation of NF-kB in Eph4 cells in response to LPS and PAM3CSK4 is demonstrated (F). D-luciferin was added at time 0, 50 and 100 min (broken lines) and luminescence signals were quantified every 5 min using SpectraMax i3x multiple detection microplate reader (Molecular Devices, CA USA). Low resolution luminesce imaging of transduced BEND cells (B) was performed using IVIS Lumina Series III (PerkinElmer Inc., MA, USA)