Table 1 Genes and primers used in this study
From: Bacillus pumilus TS1 alleviates Salmonella Enteritidis-induced intestinal injury in broilers
Target gene | Primer sequence(5’ – 3’) | Product size |
|---|---|---|
IL-1β | Forward:GTACCGAGTACAACCCCTGC Reverse:AGCAACGGGACGGTAATGAA | 98 |
IL-6 | Forward:CCTCCTCGCCAATCTGAAGTCA Reverse: GGACAGCCTATGCCAACAAG | 210 |
TNFα | Forward:ACACGACAGCCAAGTCAACG Reverse:ACACGACAGCCAAGTCAACG | 168 |
IL-4 | Forward:GTGCCCACGCTGTGCTTAC Reverse:AGGAAACCTCTCCCTGGATGTC | 82 |
HSP70 | Forward:CGGGCAAGTTTGACCTAA Reverse:TTGGCTCCCACCCTATCTCT | 120 |
β-actin | Forward:TTGGTTTGTCAAGCAAGCGG Reverse:CCCCCACATACTGGCACTTT | 100 |