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Fig. 4 | BMC Veterinary Research

Fig. 4

From: N-thiocarboxyanhydrides, amino acid-derived enzyme-activated H2S donors, enhance sperm mitochondrial activity in presence and absence of oxidative stress

Fig. 4

Representative images of hydrogen sulfide (H2S) release in porcine sperm cells supplemented with or without N-thiocarboxyanhydrides (NTAs) after 3.5 h of incubation at 38 °C. A Negative control group supplemented with dimethylsulfoxide only (vehicle); B Positive control group supplemented with the fast H2S-releasing donor Na2S 30 μM; C Samples supplemented with NTA derived from glycine (Gly-NTA) 10 nM; D Samples supplemented with Gly-NTA 1 nM; E Samples supplemented with NTA derived from leucine (Leu-NTA) 10 nM; F Samples supplemented with Leu-NTA 1 nM. Samples were stained with H2S selective fluorescent probe WSP-5

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BMC Veterinary Research

ISSN: 1746-6148

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