Fig. 6

Inhibited the apoptosis of spleen lymphocytes in KM mice infected with PCV2 at 11 d by Matrine combined with Osthole. a The IHC results of spleen showed that, compared with the normal group, the apoptosis of lymphocytes in the spleen tissue was more significant in the group of PCV2 infection group. The cell apoptosis was inhibited in each treatment group, and the group of Matrine combined with Osthole had the strongest effect (Bar = 50 μm). b-e Western blot results showed that the expression of cleaved caspase-3 and Bax was significantly increased (p < 0.05) and the expression of Bcl-2 was significantly decreased (p < 0.05). Cropped blots are displayed, the samples derive from the same experiment and that gels/blots were processed in parallel. Different letters indicated significant differences between the groups, p < 0.05