Table 2 Mean PRRSv PCR treshold cycle (Ct) values for different samples collected from sows in the PRRSV challenge study in pregnant sows. Samples were collected on different days post challenge and days post-farrow. Values equal or higher than 37 were considered as not detected (nd) and are not included in the calculation of the mean Ct values. Values between brackets indicate the ratio of positive samples by total number of samples
Mean Ct values of positive samples (number of positive samples/ number of samples) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Days post challenge | Days post-farrow | ||||||||||
Sample type | Group | -3 | 0 | 7 | 14 | 21 | 0 | 2 | 9 | 16 | 21–25 |
Faecal swab | Untreated-unchallenged | ns | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/5) | nd (0/5) | nd (0/5) | nd (0/5) |
Untreated-challenged | ns | nd (0/6) | 30.1 (4/6) | 34.6 (4/6) | 33.6 (3/6) | 34.1 (4/6) | 33.4 (1/6) | 31.7 (1/6) | nd (0/6) | nd (0/6) | |
Treated-challengeda | ns | nd (0/6) | 30.6 (3/6) | 33.3 (3/6) | 32.4 (1/5) | 33.5 (4/5) | 31.6 (2/5) | 32.4 (2/5) | nd (0/5) | nd (0/5) | |
Oral swab | Untreated-unchallenged | ns | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/5) | nd (0/5) | nd (0/5) | nd (0/5) |
Untreated-challenged | ns | nd (0/6) | 33.4 (4/6) | 35.4 (1/6) | nd (0/6) | nd (0/6) | 36.0 (1/6) | nd (0/6) | 36.8 (1/6) | nd (0/6) | |
Treated-challengeda | ns | nd 0/6) | 32.9 (3/6) | nd (0/6) | nd (0/5) | 35.7 (1/5) | 33.8 (1/5) | nd (0/5) | nd (0/5) | nd (0/5) | |
Blood serum | Untreated-unchallenged | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/6) | nd (0/5) | nd (0/5) | nd (0/5) |
Untreated-challenged | nd (0/5) | nd (0/6) | 25.8 (6/6) | 32.1 (6/6) | 32.6 (6/6) | 33.3 (5/6) | 33.4 (4/6) | nd (0/6) | nd (0/6) | 36.4 (2/6) | |
Treated-challengeda | nd (0/6) | nd (0/6) | 25.4 (6/6) | 32.7 (6/6) | 33.2 (4/5) | 34.1 (4/5) | 32.9 (3/5) | 35.6 (2/5) | 34.3 (2/5) | 31.9. (1/5) | |