Table 1 Primers of target genes analyzed by real-time PCR
Genes | Primer sequence (5′-3′) |
|---|---|
TNF-α | FW: TGCCTACTGCACTTCGAGGTTATC |
RW: CAGATAAGCCCGTCGCCCAC | |
IL-1β | FW: AATTCGAGTCTGCCCTGTACCC |
RW: GCCAAGATATAACCGACTTCACCA | |
IL-6 | FW: CAGAGATTTTGCCGAGGATG |
RW: TGGCTACTGCCTTCCCTACC | |
IL-8 | FW: GACCCCAAGGAAAAGTGGGT |
RW: TGACCAGCACAGGAATGAGG | |
LGR5 | FW: CCTTGGCCCTGAACAAAATA |
RW: ATTTCTTTCCCAGGGAGTGG | |
Lysozyme | FW: GGTCTATGATCGGTGCGAGT |
RW: AACTGCTTTGGGTGTCTTGC | |
SGLT1 | FW: TCACCAAGCCCATTCCAGATG |
RW: GCTTCTTGAATGTCCTCCTCCT | |
GAPDH | FW: ATGGTGAAGGTCGGAGTGAA |
RW: CGTGGGTGGAATCATACTGG |