Fig. 7
Effect of piperine on CFTR-mediated fluid secretion in STa toxin-induced swelling assay. 3D porcine duodenal enteroids were seeded on 48-well plate. Cells were then treated with piperine, CFTRinh-172, GlyH-101, or DIDS for 1 hour prior to stimulate with 100 nM STa toxin. (Left panel) Representative images of 3D porcine duodenal enteroids after 3 hours treated with 100 nM STa toxin (Right panel) Quantitative data of enteroid area after 3 hours treated with 100 nM STa toxin. Enteroids areas were measured at least 10 enteroids per 1 sample groups (n = 5) (**: p-value < 0.01 compared with vehicle, ##: p-value < 0.01 compared with 100 nM STa toxin)