Table 2 iELISA reaction procedure
Step | Dilution | Vol. (μL)/Well | Inc. period (min) | Temp. (°C) | Wsah × time (min) |
|---|---|---|---|---|---|
Coating | Antigen 1 μg/mL | 100 | 120 | 37 | 3×5 |
Blocking | 5% skim milk | 200 | 120 | 37 | 3×5 |
Adding serum | 1:50 dilution | 100 | 30 | 37 | 3×5 |
Adding second antibody | 1:4000 dilution | 100 | 90 | 37 | 3×5 |
Substrate | NA | 100 | 20 | 37 | NA |
Stop | NA | 50 | Within 30 min | 37 | NA |