Fig. 4From: Evaluation of the immunogenic properties of the recombinant Histophilus somni outer membrane protein 40 kDa (rOMP40)Reactivity of IgG antibodies against whole cells E.coli (a) and P. multocida (b) in ELISA. Legend: Figures show the reactivity of IgG antibodies against whole bacterial cells of E.coli (4.a) and P. multocida (4.b). The blood samples were obtained before the first immunization/injection (E0, C0), 3 weeks after the first immunization/injection (E1; C1) and 2 weeks after the second immunization/injection (E2; C2). Individual study groups are labelled with C0, C1, and C2 (white bars) and E0, E1, and E2 (gray bars). Significant differences within individual study groups (C0-C2; E0-E2) are labelled with different uppercase letters (A-B) for P ≤ 0.01. Significant differences between study groups in respective dates (C0-E0; C1-E1; C2-E2) are labelled with an asterisk (*) for P ≤ 0.01. ELISA plates were coated with E. coli (4.a) and P. multocida (4.b) whole cells. Sera were diluted 1:3000Back to article page